Sample that has not been cataloged in a genomic data repository, with a positive number of Paraoxon bacteria growth and zero Methyl Parathion bacteria growth
The sample was collected in an area known for chemical dumping (Brio Superfund Site) until stopped by the EPA for protection of the environment.
The sample was collected 7-8 inches deep next to a bayou that usually get flooded during heavy rain.
The soil sample was collected from a community garden/farmland that has been recently used to grow tomatoes, peppers, squash, onions, and cucumber. The soil was on the sandy side.
This was near the bayou of Macgregor Parkway, a park in Houston that sees a regular treatment of pesticides to fight of weeds and other unwanted organisms. Frequent flooding in the area also helps contribute to the variety of microorganisms that may be found.
The sample was collected in a wooded area where a small river was flowing through and it was a mile away from a farm. For inoculation results, the inoculation samples turned yellow in minimal media subcultures containining paraoxon (Px) which indicates production of P-nitrophenol due to OP hydrolysis. The color was brighter in CSM medium compared that in SSM medium which suggest that the bacteria prefer carbon as their food source. As for subcutlures containig methyl parathion (MP), they showed no colors after a series of inoculation.
The sample was collected near Memorial Park Conservancy. This place likely to have pesticide use or activity, because it is expected to discover organophosphate enzymes where pesticides are used, the collection of the sample was performed near an area where pesticides are ubiquitous. Following several inoculations, screening was carried out on the CMM plate to detect OP hydrolysis.
Sample of dirt collected near train tracks
The sample was collected, where plant growth was not much. There was some landscaping done on a non-regular basis which includes using pesticides. This place was also a place of interest as it is in very close proximity to chemical plants.
The soil sample was collected near the Brio Superfund industrial site that was located near San Jacinto College. This site was known for storing Petroleum and waste until EPA taken it down due to leakage of toxic waste. Contaminated soils were dug out and removed.
Soil sample collected was at a nearby neighborhood bayou where it experiences frequent flooding, which possibly contributes to the growth of various bacteria. Additionally, this area is predicted to have come into contact with pesticides to certain plantation from growing in the area.
