Overview Sample Description The collected soil sample that I have provided was obtained at the coordinates 29.7238200, -95.1137860 on August 28, 2018 at 12:41PM on a sunny day. The collected sample was taken from the East Fork Patrick Bayou nearby the Buffalo Bayou. The sample was retrieved from an 8-12 inch hole along the bank of the bayou. For week 0, 1.05g of the sample is initially inoculated into an enriched media then for week 1-5 200µL of the previous week’s inoculation culture is added to separate minimal media tubes containing Carbon Selective Media with Paraoxon, Carbon Selective Media with Methyl Parathion, Sulfur Selective Media with Paraoxon, and Sulfur Selective Media with Methyl Parathion. The best indicator for the metabolism of pesticides is then inoculated onto the enriched media for bacteria revitalization then 100μL of the enriched media with bacteria is plated onto a minimal media with MP. The plated sample is then analyzed to confirm that the bacteria is able to tolerate the Methyl Parathion compounds, but not degrade it. Date of Collection Tue, 08/28/2018 - 12:00 Semester of Collection Fall 2018 My Institution UH Results Number of Colonies of Paraoxon 0 Number of Colonies of Methyl Parathion 0 Location Geolocation for Sample 29.72382, -95.113786 Location Name East Fork Patrick Bayou Location Street Tidal Rd Location City Houston Location County Harris County Location State Texas Location Country US Location Postal Code 77015 Sample Result Classification Non-Cataloged Sample with NEGATIVE Paraoxon NEGATIVE Methyl Parathion
