This is a map of all of the ESRM samples, with each sample location represented by a pin symbol that describes the results of the analysis. A description of each pin symbol is shown below the map. You can click on a pin to view a popup that describes the sample in more detail. Clicking on the sample title in the popup displays a page with even further details about the sample.
You can filter the map according to whether the samples were positive for Paraoxon, Methyl Parathion or whether the sample was Cataloged. When a sample is cataloged that means that it has been registered in a third party database (e.g., NCBI).
Soil taken from 12 inches below frozen surface. Sample analyzed by Abigail Miller.
Soil collected from surface. Sample analyzed by Kara Morrison
Soil collected from 12 inches beneath the frozen surface. Sample analyzed by Kevin Schutt.
Soil collected from 12 inches below frozen surface. Sample analyzed by Jiacheng Wu and Kevin Schutt.
Soil collected from 12 inches below the frozen surface. Sample analyzed by Abigail Miller and Zachary Ward.
Soil collected from 12 inches below the frozen surface. Sample analyzed by Kara Morrison.
Sample taken from 12 inches below the frozen surface. Soil analyzed by Ben Hall and Breanna Laber.
Soil sample taken from 12 inches below the frozen surface. Sample analyzed by Zachary Ward.
Soil taken from 12 inches below the frozen surface. Analyzed by Abigail Miller and Kara Morrison.
The soil sample was collected from a retention basin that flooded during Hurricane Harvey.
PX MP negative due to only having growth but not hollows around it. sample cannot break down OP
The sample was collected in the Stude Park surrounded by a lot of plants.
The presence of growth on my Glycerol Minimal Media agar plate indicates I have bacterial strains that are capable of tolerating the pesticide, Paraoxon. I also have a few colonies with halos which are an indication of hydrolysis, meaning that I also have a strain that might be capable of degrading OP compounds.
Inoculation of CSM Px tube was plated on minimal media agar plate. The CSM Px tube had the most prevalent yellow coloration, indicating it may be able to degrade organophosphorus compounds; This is why CSM Px was chosen to be plated.
Found soil along a stream in Crighton Park in Conroe, Texas.
Sample collected near Stafford
Collected 8/29/2018, 7:30 AM. Soil sample was collected nearby bayou and construction site by a biking trail. Soil sample shows negative for Paraoxon under GGM- PX assay.
Located near the edge of Brays Bayou, sample was collected around 8:35 on the morning of the 10th of September, 2018.
Located by the fountain
Sample collected from: Northwest Houston from the soil near the outskirts of a retention pond.
Coordinates 29.988894, -95.475892
Colonies were tolerant when grown in PX GMM Plate, but showed no sign of OP degradation.
Sample was collected from a creek bed at a residential park.
The sample was collected by the Brays Bayou near the University of Houston.
Sample was taken from riverbed at Buffalo Bayou Park. Location was 15meters from a common running path. The site had indications that water levels were subject to constant change according to the weather. Soil sample was mostly sandlike in texture and fairly moist. Site was located under a bridge.
Tolerant of Paraoxon but not able to degrade it, and intolerable of methyl parathion. A gross colony count of around 615.
Soil Sample from Keegans Bayou
When collected, the soil sample had a dark muddy appearance.
The sample was collected at River Terrace park in Channelview Texas. The GMM plate was negative for Px degradation.
the sample was collected from my backyard
Sample was taken along the ditch line, about 8 - 12 inches deep
I had colony growth with a few cells that had Halos, indicative of OP degradation.
Sample found near a neighborhood man made pond.
The collected soil sample that I have provided was obtained at the coordinates 29.7238200, -95.1137860 on August 28, 2018 at 12:41PM on a sunny day. The collected sample was taken from the East Fork Patrick Bayou nearby the Buffalo Bayou. The sample was retrieved from an 8-12 inch hole along the bank of the bayou. For week 0, 1.05g of the sample is initially inoculated into an enriched media then for week 1-5 200µL of the previous week’s inoculation culture is added to separate minimal media tubes containing Carbon Selective Media with Paraoxon, Carbon Selective Media with Methyl Parathion, Sulfur Selective Media with Paraoxon, and Sulfur Selective Media with Methyl Parathion. The best indicator for the metabolism of pesticides is then inoculated onto the enriched media for bacteria revitalization then 100μL of the enriched media with bacteria is plated onto a minimal media with MP. The plated sample is then analyzed to confirm that the bacteria is able to tolerate the Methyl Parathion compounds, but not degrade it.
Outside of a neighborhood, in a big field with long grass and a tunnel water pipe running through it.
The sample was collected in Houston Texas.
My sample was collected on August 26th, in League City, TX 77573.
3 feet from the river
Soil sample was collected from a citrus tree in a residential backyard.16s rDNA:
The location the soil sample was taken from a vegetable farm at 3217 Spring Cypress Rd, Cypress TX 77388.
An 8-12 inch deep was dug at this location and about 10g sample was taken. It was stored in a container that protected the sample from UV sunlight. In addition the surrounding temperature was kept at room temperature.
A hole was was dug approximately 12 inches deep, right off the trail, about 8 feet from the bayou water, and approximately 20-gram soil sample was collected, then placed in a 50mL amber tube. The soil sample was a mixture of moist and dried dirt.
Soil collection from the Brazos river near a recreational park on a rainy day. Sample tolerated well in minimal medium but demonstrated no sight of hydrolysis.
Sample was collected from my home garden, which is treated with pesticides every 3 months. Regardless, no evidence of pesticide degrading or tolerant bacteria was found.
This site is near Greens Bayou, which was contaminated with DDT. There was a concrete slab inside plastic fabrication plant, upstream, that had stains from liquid dripping into the ditch. Likely from rain, but may also have carried contaminants from the plant into the ditch. Sample was taken from this ditch close to the drain by a back gate, see attached image. Sample was taken halfway down; despite being above the water table below sample was mostly mud.
Soil collected from a home garden.
The soil sample was collected from the side of Berry Bayou. A hole was dug, 6-8 inches deep, and the soil at the bottom of the hole was collected and stored into a dark tube.The sample was kept at room temperature.
soil collection from Bayou Parkland near Brays Bayou
It was muddy, and wet.
Sample was collected from a garden that is regularly treated with pesticide. However, there was no growth on the plates.
Dirt was collect at a cotton farm by hwy 99 at the corner of morton rd and harlem rd in richmond tx at 7:30pm
The area where the sample was obtained is known to have their course exposed to pesticides in order to keep the grass healthy. However no results where obtained at all. This could have to do with the that part of the golf course might be new or the course isn't as exposed to pesticides as one would think.
This soil sample was collected at Four Seasons Park near Katy area. The time was around 5:00pm on August 30, 2016. The latitude is N 29°50'25.956'' and the longitude is W 95°48'2406''.
Bacteria tolerant to PX. Several colonies showed halos around the edges which means there is a presence of hydrolysis of PX.
(Student: Alyssa Nguyen) Soil sample taken near bank of Buffalo Bayou after Hurricane Harvey - Dug about 5 inches. Had a lot of clay when obtained.
one of the agar plates has too many colonies therefore it was difficult to count but the second agar plate has many small colonies. the fuzzyiness around the colonies means degrading.
Px CSM Weeks 1-5: CYYYY Px SSM Weeks 1-5: WWTTT MP CSM Weeks 1-5: SSSSS MP SSM Weeks 1-5: WWTTT Plated on CSM agar with PX and MP. Growth with halo in both PX and MP. Preliminary Hydrolysis Test was Positive in both PX and MP. Location on Farm to Market Road 521 (approximate address 11730) in between and parallel to Willow Lake and County Road 55. Coordinates: 29.413507,-95.477886
The soil sample came out to be positive for paraoxon and was able to withstand the pesticide.
Digging a hole 12 inches into the ground and obtaining the soil in a sample container tube.
Sample was more like a mud texture, heavy rains over Memorial Day weekend. The sample was collected about 10 yards away from actual bayou water and about 8 inches below surface. It was moist, not too wet neither too dry. Its smell was very peculiar. Paraoxon (Px) Methyl Parathion (MP) CSM SSM CSM SSM WEEK 1 N/A N/A N/A N/A WEEK 2 slight yellow and turbid pronounce yellow and clear slight yellow and clear turbid white WEEK 3 slight yellow and turbid pronounce yellow and clear slight yellow and clear colorless and clear WEEK 4 slight yellow and turbid pronounce yellow and clear turbid white colorless and clear WEEK 5 slight yellow and turbid pronounce yellow and clear turbid white colorless and clear At time of screening for OP hydrolysis, there was no growth.
Muddy soil sample with unknown components.
Charles Milby park and Sims Bayou is down stream from Buffalo Bayou, where PCI Nitrogen - a fertilizer production facility. The sample bacteria were able to hydrolyze Paraoxon in the sulfur-selected medium after five weeks of dilution, transfer, and incubation.
Soil was collected from park close to University of Houston-Main Campus
The soil obtained was in my parent's garden.
The sample was taken in the backyard, near the trunk of a tree.
sample was collected about 10 yards from the receding water line, water of the bayou was about at 70% capacity during the time of collection because of a series of heavy rain falls from the weeks leading up to the collection which included a heavy flooding during memorial day weekend 2015. This meant that the location of collection was resting in standing water.
Sample was collected from a backyard garden. The area had 15 years of previous pesticide usage.
The soil sample was dark grey, sandy and smelled like gasoline/crude oil.
Found near a residential man-made pond.
This sample was obtained at the bayou that was located at the intersection off of grandparkway (SH 99) and FM 1464 in Sugar Land, Texas.
Sample was taken from a small floral garden. Soil was dry, light brown in color, and full of small rocks.
Over the course of a few weeks, it was evident that no result was going to be obtained for MP. By the 5th week, all tubes were close to becoming clear with a very slight yellow color.
The location was in Buffalo Bayou between Sam Houston Tollway and Independence Pkwy. The soil was collected near the Old River along Launch Rd (Old River Terrace, Channelview TX). The approximate latitude and longitude coordinates are: 29°46'54.2"N 95°06'03.3"W. The soil was collected on August 30, 2016 around 9:30 PM. The collected sample was clay-like and moist because of the recent rainy weather.
Ballester Fishing Area - La Porte
20g of soil were obtained from Alex's garden. Soil has been exposed to pesticides for at least 12 months.
Paraoxon positive, MP negative
Sample taken from a Garden Center which use pesticides in all their plants.
This sample was collected from farmland, which was thought to contain OP hydrolyzing bacteria. The bacteria isolated from the sample, which was collected during the spring 2017 semester, was paraoxon tolerant but not capable of OP hydrolysis.
The sample was a mixture of sand and dirt. It was taken from an area that was affected by flood water. Once grown on a Px plate there was many tolerate bacteria and a few bacteria colonies that degraded the pesticide with soft edges.
I collected a sample that was at the waters edge where many birds had been so the top layer was mostly white bird poop and the lower levels were clay with colorful bands of sand and a little bit of algae that was floating atop it. It smelled like Galveston beach, which is ironic (or not) because Bray's Bayou flows right into Galveston Bay.
This sample contains bacteria capable of hydrolyzing Paraoxon(Px) present in pesticides. After the transfer of 100microliters into MM plate, a total count of approximately 540 colonies was observed. Number of Tolerant colonies: approximately 530 Number of Degrading colonies: approximately 10
I collected this soil from the cotton field. I thought this would be great idea because cotton field is one of the farms where farmers use lots of pesticides and insecticides.
Soil collected from the Brock Park Municipal Golf Course
The soil was collected from Steinhagen Farms on crop growing fields. After many weeks of sampling and screening on minimal media; the sample was found to be Paraxon Positive; and Methyl Parathion Negative (yellow).
The soil sample was collected in a drainage ditch in the Katy area
sample was taken under bridge in the middle of Steep Bank Creek
Sample was taken from a backyard in a residential home in Houston. The sample was positive for Paraoxon tolerance and was able to degrade the pesticide and OP compounds.
soil sample, texture was very fine
The overall soil sample was dry, but there were some damp soil. The soil was inoculated over a period of 5 weeks. The overall test results were all negative
My soil sample long description - about how and where the sample was taken and any special circumstances
Sample was taken after Hurricane Harvey at Buffalo Bayou. Soil Sample was mostly wet sand/dirt mixture.
Soil sample collected from backyard (side of the house near the main water line). Coordinates: N 29°40.058' W095°35.364' Even though there were signs of pesticide degradation detected in media with Paraoxon (Px) pesticide present, sample did not grow after inoculation into enriched media and overnight incubation. Sample was a false positive.
Soil sample collected from the strawberry field at Froberg's Farm. Sample was taken at 9 am on September 21, 2012. Results over 5 weeks of isolationg and growing the bacteria from the sample. Paraoxon (PX)& Methyl Parathion (MP)are insecticides CSM and SSM are medium. Y = yellow, T= colorless, C = slight yellow and turbid, S=slight yellow and clear, W=turbid white W# is week number PX + CSM = W1-Y, W2-Y, W3-Y, W4-Y, W5-Y PX + SSM = W1-W, W2-T, W3-S, W4-Y, W5-S MP + CSM = W1-W, W2-T, W3-T, W4-T, W5-T MP + SSM = W1-C, W2-C, W3-T, W4-T, W5-T Around 15 colonies of Paraoxon grew on CSM agar plate but no halo was showed on colony.
Soil Sample was collected from a farm outside Rosenberg. The farm seemed freshly sown because the soil was turned over and some crop seem to have been planted.
An 12 inches hole was dig and soil was obtain. The soil sample was first inoculated in an enriched media and incubate at 37 degree Celsius. The soil containing media was then pipettes in minimal medical containing paraoxon and methyl parathion. Again was incubate for 7 day at 37 degree Celsius. This process was repeated until week 5. Then plated onto minimal agar with pesticide to observe for OP hydrolysis.
Bacteria collected and cultivated.
Retrieved near Brazos River
the sample was collected near the river.
Brown clumpy soil. no scent. Citrus fruit. 1 foot beneath the surface.
Sample taken from garden with hopes of finding organophosphorous degrading bacteria
Sample collected at the surface, dry and the land was not used to grow anything. Did not expect to find pesticide degrading bacteria.
Soil Sample: Sandy soil slightly moist from the bank of the bayou located inside of the park with debris spread within.
Sample was collected from the banks of Brays Bayou next to the Brays Bayou Greenway Trial. The sample was dug up from about 6-8 Inches deep. The sample was collected after a flood therefore the soil sample was very damp and had the texture of clay.
My sample was very moist prior to inoculation. My bacteria was plated on CSM agar with Paraoxon and developed no significant growth.
The soil sample was black, and it was collected from a cotton field on September 1st, 2014. It tested positive for paraoxon and negative for methyl parathion. After ten days of incubation, it was observed too many to count (TMTC) colonies on the minimal agar plates. However, I counted approximately 22,069 colonies.
This sample was taken from a garden in a residential area near community tennis courts in Misty Meadow Ln, Houston, TX.
It was obtained from a muddy region by the technology annex building
Collected in Sugar Land, TX. Degradation of Bacteria on OP Compound Paraoxon
Old Richmond Rd and Copano Bay Drive. It came from a little flower patch.
Sample taken from a crop field currently in use in Alvin, TX.
This sample was collected from the nearby shore of Rio Grande, the main river that divides the US with Mexico along Texas. It is noted that a significant amount of contaminants run down this river, making it a very suitable place for data collection. The sample was taken from the US side in a nearby zone close to the international Bridge Juarez Lincoln. During the incubation and transferring process of 5 weeks, the sample displayed increased activity in Paraoxon CSM enriched media with a noticeable yellow coloration, whereas the rest of the tubes did not show any activity and progressively faded into a complete clear coloration. After plating and growth the whole plate was completely covered and made colony count not possible, however prominent white/yellow colonies can be seen which are approximately 200. These colonies are possible zones of hydrolysis of bacteria that is expressing an OPH gene.
Soil sample collected from the bayou behind the apartment complex Bayou Oaks from University of Houston Housing. Sample collected from 12 inches deep ground for better chances of getting a better result.
Mound of dirt collected from about 3-6 inches deep near
Muddy soil sample
Sample was milk chocolate brown, with some moisture present.
The soil sample was dug from an area close to the big lake of the Kickerillo-Mischer Preserve in Houston. There was dead fish all over the margins of the lake because Harvey had brought fish from other areas (farms) that were not surviving to that particular environment.
The soil sample had a little moisture content when collected from the farm.
The sample was dry and clumpy dirt resembling nestle chocolate powder. It was collected from Theiss Farms. The sample tested negative for Organophosphorus degradation.
front yard of a house in farming area in Jersey Village (Houston, Texas). 8 inches deep. The sample looks dark and moisture when i got it fromt he yard.
Soil collected from a home garden and pesticides are used consistently in this garden. 10g of Soil sample was collected into 50mL amber tube
Soil was just a brown color, with a bit of a red tint. Did not really smell out of the ordinary, just smelled like regular dirt.
Soil collected from a small runoff from Brays Bayou located on Chimney Rock near South Braeswood.
The sample degraded Paraoxon and used it for a carbon source and a sulfur source. It showed no signs of being able to degrade Methyl Parathion. It did seem slow to adapt to relying solely on the pesticide as a food souce, but once it adjusted it became every adept at it.
Soil collected from median lane between two driveways located near the building of MD Anderson Cancer Center Proton Therapy Center on the street of Old Spanish Trail, Houston, TX
The isolated bacteria degraded OP compound(s) on both MP & Px Media
The sample had bacteria that were able to degrade organphosphorous hydrolase found in Paraoxon pesticide
Sample was taken from behind a home garage because it was suspected to have been treated for ants with strong chemical insecticides.
CSM with paraoxon was yellow. Growth halo and tested positive. There was about 14 colonies on the plate
Sample was collected from field near North Main Street, Pearland TX. Although there was a positive result for paraoxon hydrolysis, there was no growth when added into the enriched media and incubated. There were a lack of microorganisms for the sample. Coordinates: 29° 35’ 30’’ N 95° 17’ 12’’ W
Sample tested positive form paraoxon with about 26 white colonies. Halos are present which show sign of degradation.
Muddy sample along the waters edge
sample was from the buffalo bayou area
The colonies were grown on CSM agar + Paraoxon(100mg/mL conc.) @ 1uL/mL Notes 1:100 Dilution (in SOC, 200uL plated) (in SOC, 200uL plated) 11 colonies 1:1000 Dilution (in SOC, 200uL plated) (in SOC, 200uL plated) 2 colonies Total: 13 colonies
My soil sample was tested for OP biodegradable bacteria. The pesticide used was paraoxon. Testing was done within a 5 week period and results of the tests were negative.
taken from bear creek park in Houston, Tx, soil taken from approximately 12 inches into the ground,
Sample was taken from John P. McGovern Centennial Gardens in Houston, TX.
The soil sample was collected from the small garden where usually use pesticide
This sample was collected near a drain at Cullen park which is a part of Addicks Reservoir, after Hurricane Harvey
Soil sample collected near the Historic Santa Anna Capture Site
Sample collected in a residential area.
Soil sample was taken from Froberg Farm on August 30th, 2014 at around 11 am. At the time when soil sample was taken, no produce were seen growing on site. The farm land was being prepared for planting new batch of seeds. After the weekly inoculation, an average of 19 colonies were seen growing on the agar plate. The bacteria are tolerant to withstand the toxic environment however they are unable to degrade the organophosphorus compound present in the pesticide
Sample was collected on a day after which it had rained.
Sample Collected near at the gated boardwalk area near the ship since the other side was closed off closer to the waters. Highly polluted area(cigarettes, paper, trash, food, etc)
Got soil from buffalo bayou, by the battle of Texas Historic Site
The sample was taken from cullinan park in January after a stormy day when the soil was still wet.
Sample was collected 8 feet from the fence line.
Paraoxon hydrolyzing bacteria are present. Showed negative results for Methyl Parathion degradation.
The sample was taken from the backyard near the trunk of a tree.
The soil was collected from a flower bed. The soil was soft and moist.
Soil was collected from Mason Park near a marsh. After liquid media culturing and screening on a minimal agar plate, the sample was found to be Paraoxon Positive and Methyl Parathion Negative.
I got my sample from a bayou by the Fort Bend County fairground in Rosenberg.
The sample was mostly solid with a little bit of twigs and sand mixed in.
This soil sample was taken from within the Barker Reservoir after Hurricane Harvey.
My sample appears to be from farm land. My starting sample was not as soft as other student's samples. The soil I collected had a texture more like clay than regular soil.
The collected soil was from Froberg Farm, where several vegetables along with strawberries are grown. The soil was dark brown in color and heavily moistened. During the process of isolating and growing the bacteria present in the soil sample, I have observed color changing patterns in a weekly basis on specific combination of medium and insecticide. The results are as follows: Week 1, 9/21/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = yellow color and lightly turbid, (C) Methyl Parathion (MP) + CSM = pronounce yellow color (Y) Methyl Parathion (MP) + SSM = yellow color and lightly turbid, (C) Week 2, 9/28/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = Colorless (T) Methyl Parathion (MP) + CSM = yellow color and lightly turbid (C) Methyl Parathion (MP) + SSM = Colorless (T) Week 3, 10/05/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = Colorless (T) Methyl Parathion (MP) + CSM = yellow color and lightly turbid (C) Methyl Parathion (MP) + SSM = Colorless (T) Week 4, 10/12/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = Colorless (T) Methyl Parathion (MP) + CSM = yellow color and lightly turbid (C) Methyl Parathion (MP) + SSM = Colorless (T) Week 5, 10/19/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = Colorless (T) Methyl Parathion (MP) + CSM = yellow color and lightly turbid (C) Methyl Parathion (MP) + SSM = Colorless (T) Around 80-90 colonies of bacteria were successfully isolated from the soil sample and grown well on the CSM Agar media with Paraoxon pesticide
Soil collected from JRN Nursery.
Soil was collected in a backyard in a suburb in Killeen, Texas. The soil was collected close to the house as the house gets regularly sprayed for bugs.
PX and MP screening for OP hydrolysis
Found near a gulf course by the Steep Bank Creek
Soil Sample was collected from cotton field on Benton Rd in Richmond, Texas
A soil sample collected from a grass farm in Crosby Texas.
The soil sample presented OP degradation
After 5 weeks of testing by progressively diluting the bacteria sample in SSM (Sulfur selective media) and CSM (Carbon Selective media) in both Methyl Parathion and Paraoxon condition it was determined what type organophosphatase activity is found in this bacteria sample after growth in a media rich dish. SSM consistently had more turbidity than the CSM (Carbon selective media) , but only the PX (paraoxon) samples ever had a breakdown to form the yellow color p-nitrophenol product in both, turbidity or no; MP, or Methyl Parathion media NEVER had yellow coloration. This trend stayed throughout, but eventually only the CSM had a yellow color in the 5th week trial with almost no turbidity in any sample. The final trial's SSM PX should still have had a yellow color, but it may have been due to a too great dilution of the soil bacteria by this point that it could not break down the Paraoxon, whereas the CSM-PX had just enough to break down the Paraoxon. However, my bacteria in particular grew best on an SSM based on turbidity consistently staying higher in the SSM tubes. This means that it is a bacteria that breaks down Paraoxon in order to obtain its sulfur or carbon products; the slightly higher level of turbidity in the SSM media throughout the 5 weeks may indicate the bacteria prefers sulfur for growth.
CSM with Paraoxion was yellow in color, had some growth when plated on CSM agar, tested positive
Sample taken from rural area in South Houston on FM 2234. Exhibited a positive reaction with Px + SSM. Showed evidence of OP hydrolysis.
An unofficial count of the colonies yields an estimation of about 79 total colonies, with 51 OP degrading colonies, and 28 OP tolerant colonies.
Grew well when inoculated with CSM Paraoxon. Plated on CSM agar with Paraoxon. Plating results showed growth with halo. Bacteria sample tested positive for Preliminary Hydrolysis Test.
Approximately 15 grams of moist Soil Sample was collected by the Miller Outdoor Theatre.
Soil Sample was taken from Blessington Farm. Originally thought to be laced with pesticides, but found out from the owners that minimal pesticides were used on their farms. Additionally, flood water submerged the farm back in April might had an impact on the bacteria community. The spot chosen on the farm was known to be with "most" pesticides, the soil collected was clay-like and smell like manures.
Muddy soil sample
Many small colonies. No hydrolysis is presented on the sample. No Methyl Parathion growth was presented on the plate.
Soil collected from hole approximately 10 inches deep three days after flood waters receded.
Moist and dense soil extracted from Bayland Park Dr near a baseball field from an ~8 inch deep hole in the ground.
My sample contains approximately 80-95 positive tolerant colonies. The tolerant bacterial colonies weakly or incompletely degraded CSM media containing Paraoxon pesticide to grow and survive.
Grayish Brown and dry soil sample. It was collected from the land where it was farmland before.
was wet, clumped, and muddy.
Soil sample was collected on the banks of the Buffalo Bayou. There were men fishing and a great deal of trash (beer cans, used condoms, etc) in the area.
moist soil off the banks of Willow Waterhole Bayou offshoot, clumpy and clay-like
Halo was present surrounding Bacterial colonies in CSM- Px Agar plate indicating bacteria was able to hydrolyze organophosphorus compound and giving a positive result.
The soil bacteria sample was collected from the Japanese garden within Hermann park.
The soil sample was actually hard like clay and was very dark when I had collected it from the park
Muddy sample of soil
The soil sample was taken taken from a local park located in SugarLand. A 8-10 feet hole was dug into the ground and a mixture of wet and dry soil was collected in put into the soil collection tube. Over the course of 5 weeks it was noted that the Sulfur Selective Media with the Paraoxon was the best at showing pesticide degradation in bacteria. It was noted in week 4 and week 5 of the experiment that the the SSM+PX tube turned orange then, burnt orange. In the agar plate, it was noted that the PX resulted in two types of bacteria, the larger bacteria around the outside was able to hydrolyze and the bacteria towards the center was only able to tolerate the paraoxon.
this sample was collected from a bayou with the adress of 16600-17080 Beechnut st Houston TX 77083 on 09/08/2012. the sample was moist.
Kevin Smith sample 1
Week 1 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-Yellow Mp SSM-Slight Yellow Week 2 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-White Mp SSM-Slight Yellow Week 3 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-White Mp SSM-Turbid White Week 4 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-White Mp SSM-Turbid White Week 5 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-White Mp SSM-Turbid White
This garden grew crops the family ate from daily so it is very likely that they used very minimal pesticides to treat their soil. Sample was moist and dark and collected on a sunny day at approximately 11:30 AM. Sample was not further processed due to lack of evidence of strong hydrolysis.
Soil sample with unknown composition
Sample was collected after hurricane Harvey when it was still very wet.
Soil was collected near a sewer in a ditch behind HEB. The bacteria were found to be tolerating the paraoxon pesticide.
Portico at West 8 apartments
Growth of pesticide resisting bacteria colonies in Paraoxon and Methyl Parathion. In Paraoxon plate degrading bacteria is present. In Methyl Parathion plate tolerating bacteria is present.
Park is regularly treated with pesticides and other chemical agents.
Soil collected from Tom Bass Regional Park near Clear Creek.
Soil Sample was collected from Atkinson farm that grows strawberries. No growth of bacteria was observed on minimal agar plate. The bacteria sampled were not able to withstand or tolerate the pesticides.
The soil was taken on September 1, 2014 around 12pm. The soil was a little wet due to the raining the previous day. The soil was gathered on the right side of the farm when coming in from the parking lot. CSM- organophosphorus is used at carbon source for bacteria culture/growth SSM- organophosphorus is used at sulfur source for bacteria culture/growth Px - Paraoxon MP- Methyl Parathion Inoculations by week Week 1 CSM (Px): light brown and turbid SSM (Px): soil color CSM (MP): light brown and turbid CSM (MP): soil color Week 2 CSM (Px): slight yellow and turbid SSM (Px): slight yellow and turbid CSM (MP): slight yellow and turbid SSM (MP): slight yellow and turbid Week 3 CSM (Px): yellow and clear SSM (Px): colorless CSM (MP): colorless SSM (MP): colorless Week 4 CSM (Px): yellow and clear SSM (Px): colorless CSM (MP): colorless SSM (MP): colorless Week 5 CSM (Px): yellow and clear SSM (Px): colorless CSM (MP): slight yellow and clear SSM (MP): colorless Result There was no culture growth observed in enriched media so minimal media agar was not performed.
This sample was collected from a creek in Cypress, TX next to farmland. I chose this location due to the vast amount of farmland in close proximity. I felt that water in the creek from run off would have pesticides in it because of the farms, thus the bacteria near the creek would be exposed to pesticides.
Soil Collection from the Location: Pasadena Refining System, Inc located at the Houston Ship Channel. 29°43'07.3"N 95°12'37.5"
Soil collected from Channelview TX off of Market street near the bank of the San Jacinto River
Sample collected from a little away from a natural gas plant. Just on the outter edges of an agricultural field.
My sample was collected from the front lawn of the home at address 1017 Brookhollow Drive, Deer Park, TX 77536; my place of residence. The city of Deer Park has trucks spray pesticides meant to control mosquitoes every Mon-Thur during the mosquito season. This practice has been in use since the late 80's, and could have helped with the creation of pesticide-degrading bacteria.
Soil was taken from a strawberry farm, the soil was light brown and slightly dry.
A soil sample was collected near a guava tree from a residential backyard. The sample was positive for paraoxon tolerance.
This sample was taken from the Walmart garden center, where they house plants and other botanical forestry
Clay like texture to the soil and it was wet
Soil sample of about 10-20 grams found behind house, in a bayou that gets flooded occasionally.
sample collected along sidewalk of Sabine Park, after Hurricane Harvey Flood.
Sample was collected by the banks of the buffalo bayou. Because of the recent hurricane Harvey, the bayou was overflowed and some soil might have been washed out.
The sample was collected from a strawberry farm in Fresno, TX. The sample tested positive for Paraxon and yielded tolerant colonies.
sample tested positive for CSM-Px pesticide.
The MP samples did not show strong evidence of hydrolysis at all. MP plates did not show any growth 29°54'49.1"N 95°42'13.3"W
Soil was collected from a flower bed in Cypress, TX 77433
The bacteria sample collected was able to degrade the PX pesticide, but was unable to degrade the MP pesticide
Soil Sample from Mary Jo Peckham Park in Katy, Texas.
This soil sample was collected from the banks of Brays Bayou that recently might have overflowed due to Hurricane Harvey.
Collected from the backyard of my home.
No significant color changes were found after 5 weeks of inoculation in Methyl Parathion and Paraoxon.Sample was taken 5 meters into cotton field immediately northwest of the Student Parking Lot.
Soiled sample collected from a field used to grow vegetables on Stuebner Airline Rd.
Soil collected from the banks of McGovern Lake outside of the Houston Zoo.
Sample taken from bank of San Jacinto river near market street.
Soil collected from a former cotton farm in Rosenberg, TX.
Collected in or near an area that was cultivated for rice 50 years ago.
The sample was acquired from a private church garden in Northwest Houston from an area that had recently been planted with corn and other harvest crops nearby. The sample was positive for Paraoxon tolerance and was able to degrade (hydrolyze) the pesticide and OP compounds.
Soil sample from front yard in a farm house (10 inches depth). the soil is very moisture and dark.
Sample taken from a park that had water from the Old River, that connects to the San Jacinto River.
Soil sample was collected from a cotton farmland located in the Rosenberg area of Texas.
This is a community farm where pesticide is not used. Approximately 100 bacterial colonies growth seen on agar plate with Paraoxon. Half of them appeared white with a halo ring in the surrounding and the other half appeared white. There is indication of bacteria that tolerate Paraoxon and bacteria that degrade paraoxon in the soil sample.
Soil collect from Shadow Hawk Golf Club
Sample was obtained at a home garden it showed Paraoxon tolerance.
This sample was collected in a public park by a bank of along the San Jacinto River
The soil sample was taken from dirt that was slightly wet due to rain the previous day. The soil's location is near a river source, but also near a residential area.
Sample was top layer of clay and middle layer of sand. The sample was also very muddy.
a small soil sample was collected from the Mint beds in the botanical garden across from Miller Outdoor theater.
Approx. 10–20 grams of soil was collected at a depth of approx. 20–30 cm from a grass farm in Crosby, TX on January 24, 2016. The sample was stored in an opaque 50 mL tube and kept at room temperature (approx. 25ºC). The soil at the collection site was wet after a day of rain.
Soil was collected at a recreational park where kids play and people exercise. There is also a dog park.
Tolerant of Paraoxon. Intolerant of methyl parathion. 2 paraoxon hydrolyzing colonies.
Sample was taken from a small cotton farm that used pesticides. Bacteria was found to tolerate Paraoxon but not degrade it.
The sample is collected from cotton field. The sample was positive for Paraoxon and negative for Methyl parathion.
Date: September 21, 2011 Week 1 Paraoxon (Px)+ CSM = So Paraoxon (Px)+ SSM = So Methyl Parathion (MP) + CSM = So Methyl Parathion (MP) + SSM = W Date: September 28, 2011 WEEK 2 Paraoxon (Px)+ CSM = Y Paraoxon (Px)+ SSM = S Methyl Parathion (MP) + CSM = S Methyl Parathion (MP) + SSM = W Date: October 05, 2011 WEEK 3 Paraoxon (Px)+ CSM = C Paraoxon (Px)+ SSM = Y Methyl Parathion (MP) + CSM = W Methyl Parathion (MP) + SSM = S Date: October 12, 2011 WEEK 4 Paraoxon (Px)+ CSM = T Paraoxon (Px)+ SSM = Y Methyl Parathion (MP) + CSM = W Methyl Parathion (MP) + SSM = C Date: October 19, 2011 WEEK 5 Paraoxon (Px)+ CSM = Y Paraoxon (Px)+ SSM = Y Methyl Parathion (MP) + CSM = W Methyl Parathion (MP) + SSM = C Legend So- same color as soil inoculate W- turbid white T- colorless Y- Pronounced yellow color S- Slight yellow color C- Yellow color and lightly turbid
A sample was taken from a home garden. it was then tested to see if pesticide degrading enzyme were found.
Muddy soil sample with unknown composition
sample taken from a Landscaping, Nursery and Tree farm in Pasadena Texas.
Collected in a home garden.
Was clumpy, wet soil from a ricefield.
Soil Collected from Sugarland Park NN
Soil Sample taken from a residential backyard garden in Friendswood, Texas, which is maintained by the owner to grow various vegetables and spices.
The soil sample was taken from Froberg's Farm, where pesticides are used. The bacteria from the Week 5 CSM+Px test tube were able to tolerate Px but not degrade it. The growth on the plate was very weak, resulting in approximately 20,208 colonies.
McGovern Centennial Gardens at Hermann Park was chosen for soil sampling because it is a community garden in which consumable products are grown. The nature of the produce being grown and the location were also factors that were taken into consideration.
Soil sample from River Green Park in Katy, Texas (10 inches deep). The soil is very moisture and dark.
Very tiny colonies grew on the agar (~8478), but there was no evidence of Px hydrolysis based on the edges of the bacterial colonies. This means that the bacteria can tolerate the Paraoxon pesticide, but not degrade it.
Collected the bank of Greens Bayou near the underpass of the Green River Drive bridge. Hurricane Harvey had hit Houston recently.
The sample was collected from a ditch on the corner of Liberty Dr and Julie St. Mosquito trucks drive by and spray pesticide every week during the summer months.
The sample was taken from the soil in a front yard in South Houston. The sample tested positive for Paraoxon, producing tolerant colonies.
A small soil sample was collected on a plot of farming land on Fernglade Drive near 4033 Gladeridge Dr, Houston, TX 77068.
Sample taken from landscaped garden plot near fountain in Buffalo Bayou Park.
Sample taken from the edge of the White Lake in Cullinan Park.
The soil sample was collected from a park. The sample was tested postivite for Paraxon and bacteria tolerated the toxic environmnet.
The soil sample was collected from garden at home that has frequent pesticides
By the memorial swimming pool, in front of a tree. The dirt was collected from 10 inches deep. Dirt was semi-moist and dark in color.
sample was obtained from golf Course which resulted in dgredgation of OP
Sample was taken from a 3 acre farm that has been in use as farm land since 2012.
Soil sample was collected at the banks of the Buffalo Bayou in Houston Tx. Because of the recent hurricane Harvey, the bayou overflowed, washing out some soil. This may have an impact in the results of the experiment.
My soil sample was collected from my backyard. We occasionally treat for insects. The bacteria in the sample was paraoxon positive and methyl parathion negative.
Very small colonies, non-clustered, white in color, numbering around 80 total. No immediate signs of clearing or degradation to indicate Paraoxon hydrolysis; however, there is sufficient evidence of growth.
i have collected a soil sample from a flowerbed located in the Gulfgate (620 and I45s)
The soil sample was collected from a residential ranch in Dayton, Texas that used to have an area for vegetation growth.
The bacteria collected were inoculated in both Methyl Parathion and Paraoxon for 5 weeks after allowing it to grow in a LB broth for a week. The Methyl Parathion samples showed no signs of life by week 3, but the sample was able to grow and digest Paraoxon. Turbidity was observed in all of the sample tubes for the first 2 weeks.
Sample was collected to test for hydrolysis of op compound
First Paraoxon Plating 88 was at a 1:100 200ul sample Second Paraoxon Plating 16 was at a 1:1000 200ul sample
SAMPLE WAS WET AND THE FARM WAS IN OPERATION. THEY GREW FRUITS AND VEGETABLES AND SOLD TO CUSTOMERS.
Sample was taken from a residential that have been treated for ants with strong chemical insecticides.The agar plates has a large number of very small colonies.
A sample of soil was collected from Cimarron Elementary. It was tested for OP degrading bacteria and produced negative results.
The cafe has a garden behind the shop. This sample is collected from that location
The soil sample was collected from Texas Avenue Park located in Webster, Texas. The sample was put into tubes containing media with two different organophosphorus compounds, Methyl Parathion and Paraoxon. The microorganisms were able to degrade Paraoxon, but not Methyl Parathion.
A large cell clump covering in the plate. Some hydrolysis is presented on the sample. No Methyl Paraxon growth was presented on the plate.
The sample was taken from Buffalo Bayou Park after heavy rainfall.
The location from where the sample was taken from had previous exposure to pesticides, and was taken from the backyard of a garden in the Northwest Houston Area.
Sample collected from 1620 N Houston Ave, Humble, TX 77338. Some colonies were very large in diameter compared to other students' results. The ring of hydrolysis is very small.
The soil sample was collected from a vegetable garden in my backyard. The soil sample was dry and crumbly. The results were negative for degrading bacteria.
Soil was extracted from ~10" under ground.
The sample was mostly dry, but some parts were wet. The sample showed no sign of hydrolysis therefore was not inoculated into rich media and was not plated onto a plate
The soil sample was collected on August 27, 2016 from Atkinson Farms. The plated sample displayed positive growth after exposure to Paraoxon and negative growth after exposure to Methyl Parathion.
Soil sample collected from a bank on the San Jacinto River Bay near the Fred Hartman Bridge. Spring 2018
I collected my sample from a corn maze, the ground was wet due to raining. I dug ground about 6-7 inches and collected sample. I stored sample in a dark tube and kept it in mild environment.
Tommy Lo Week 1 Paraoxon Methyl Parathion CSM PSM SSM CSM PSM SSM N N N N N N Week 2 W W W W W W Week 3 T T T T T T Week 4 T T S T T T Week 5 S S C W T T
Sample taken from Memorial Park Golf Course located at 6051 Memorial Dr. Houston, TX 77007. U.S.
The Soil Sample was collected in Channel View river bank by a public park around 5:27pm at 8/26/2016. The longitude and latitude was 29°46’57”N 96°6’9”W. The soil consistency was dark grey and very clay-like.
There was growth on the plate and few halos, so it is deduced that the bacteria is able to tolerate the pesticide and not necessarily degrade it. This could be evidence that a degrading bacteria was missed along the process.
Sample was collected from a small plot of soil used communicably by the apartment complex residents.
Soil sample was collected at 9:10 AM on August 27, 2010
Week 1 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. Y C N N N N Week 2 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. W W W W W W Week 3 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. S T T S T T Week 4 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. S T W S T T Week 5 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. S T T S T S
Very clay like, thick and adhesive. Plenty of moisture
Muddy sample with unknown composition
Sample positive for paraoxon hydrolysis. Sample negative for methyl parathion hydrolysis
Dirt was retrieved on a cliff near the river, the dirt was moist since it had rained a day or two before it was collected.
Soil sample collection plated on CSM agar for the viewing and identification of degrading organophosphorus bacteria.
The sample was obtained near the Sweetwater Cypress Golf Course on September 3rd, 2012.
This sample was collected from a farm with freshly sewed soil from Rosenburg, Tx.
Soil sample obtain from a golf course nearby cinco ranch katy tx
The soil sample was collected from River Terrace Park on Lakeside Drive in Channelview, Texas.
Sample was collected from the edge of a lake between a suburban and museum of natural sciences.
Methyl parathion and paraoxon - negative results for all inoculation weeks. Paraoxon Methyl parathion CSM PSM SSM CSM PSM SSM Week 1 N N N N N N Week 2 W W W W W W Week 3 T T T T T T Week 4 T T T T T T Week 5 T T T T T T
Paraoxon (PX) Methyl Parathion (MP) CSM SSM CSM SSM Week 1 So So So So Week 2 T T T T Week 3 T T T T Week 4 T T S S Week 5 Y T S S Negative results for both Paraxoan and Methyl Parathion degrading bacteria.
Soil sample was collected from irrigation ditches in a flower bed.
Soil sample collected near the San Jacinto river.
Negative results for paraxoan and methyl parathion digesting bacteria.
The soil was light brown and dry.
testing soil sample to see if it has signs of pesticide degrading bacteria in it.
Soil collected at a soccer field in southwest Houston.
Soil collected from a golf course
CSM MP results gave positive results from the beginning about degradation. CSM PX began to show slight degradation around the week 3 of sample transfer. Slight halo effects on the edges of the plate indicates OP hydrolysis has taken place. SSM MP and PX have shown negative results of degradation from the initial stage. In comparison to other members, I did not have any positive results for both SSM Mp/ Px.
Collection of soil sample to test for pesticides, It was collected from my garden where I grew carnations.
The soil was collected at the Kingwood vegetable garden in Kingwood TX.
Soil sample was collected from brays bayou biking trail. It was collected around 2:30 AM. There was a 12 inch deep hole digged to retrieve it.
Positive for Paraoxon and negative for Methly Parthion
This sample was collected near a small body of water which was flooded during Hurricane Harvey. The sample was Px positive in both CSM and SSM and negative in both MP medias.
Sample displayed tolerance for Methyl parathion on CSM.
It was collected in farm on the country side of Bandera county, San Antonio, Texas. The soil sample was collected from a organic farm where they grow tomatoes, chilies, etc. The soil was dry and hard to found a moist collection. The hole were the soil was collected was about 8cm deep. Sample was taken to the lab 4 days after collection. It was kept in room temperature all the time.
Soil sample was collected on the banks of the Buffalo Bayou. There were men fishing and a great deal of trash (beer cans, used condoms, etc) in the area. Dug about 10 inches down and collected sample in an amber tube and kept at room temperature.
The soil sample was collected on September 5, 2012. It was continuously stored at room temperature.
Soil sample taken from Froberg's Farms.
The soil was collected from farmland in La Porte, Texas where many crops have been grown. I dug a hole approximately 8 inches deep to collect the soil and kept the soil in an amber tube at room temperature to avoid the exposure of sunlight and heat.
My sample was positive for Paraoxon but negative for Methyl Parathion. In the 5 week subculturing leading up to platting there was reason to believe that there was degradation of Paraoxon and Methyl Parathion till week 3 when all yellow color faded from the Methyl Parathion tubes.